Current Issue : April - June Volume : 2020 Issue Number : 2 Articles : 5 Articles
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.\nFructus polygoni orientalis (FPO) is widely used in clinical practice in China, especially in treatment of liver diseases including\nviral hepatitis, liver fibrosis, and liver cirrhosis. However, its pharmacokinetic (PK) alterations in liver fibrotic rats have rarely been\nreported. To study whether taxifolin, one of the main flavonoids in FPO can be absorbed into blood after oral administration of\nFPO extract and to compare the differences in pharmacokinetic parameters of taxifolin to normal and liver fibrotic rats induced by\nporcine serum (PS), a UPLC-MS/MS method was developed and validated for determination of taxifolin in rat plasma using\npuerarin as the internal standard (IS). All validation parameters met the acceptance criteria according to regulatory guidelines.\nThe results indicated that after treatment of rats with PS alone for 12 weeks, the liver fibrotic model group was built successfully.....................
In Chinese medicine, the effect of promoting blood circulation and removing stasis could\nbe enhanced after Chuanxiong Rhizoma is processed by wine. However, the relevant mechanism\nremains unclear. In this manuscript, a rapid and sensitive quantification method employing\nultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was\nestablished and validated to simultaneously determine butylidenephthalide, ligustilide, senkyunolide\nA and ferulic acid in rat plasma after oral administration of raw Chuanxiong Rhizoma (RCR) and\nwine-processed Chuanxiong Rhizoma (WCR) respectively. All analytes were extracted from plasma\nby proteins precipitation with methanol������...
This study optimized the preparation of electrosprayed microspheres containing leuprolide\nand developed an in vitroâ??in vivo correlation (IVIVC) model that enables mutual prediction between\nin vitro and in vivo dissolution. The pharmacokinetic (PK) and pharmacodynamic (PD) study\nof leuprolide was carried out in normal rats after subcutaneous administration of electrosprayed\nmicrospheres. The parameters of the IVIVC model were estimated by fitting the PK profile of Lucrin\ndepot® to the release compartment of the IVIVC model, thus the in vivo dissolution was predicted\nfrom the in vitro dissolution. From this correlation, the PK profile of leuprolide was predicted from the\nresults of in vivo dissolution. The IVIVC model was validated by estimating percent prediction error\n(%PE) values. Among prepared microspheres, an optimal formulation was selected using the IVIVC\nmodel.........................
Mangiferin (MG) is an active component in natural medicines, and various studies have been reported on pharmacological effects,\nbut the low solubility and bioavailability of MG limit its wide application. The aim of the present study was to investigate the\npharmacokinetic profiles of mangiferin (MG) and mangiferin monosodium salt (MG-Na) in rat plasma by UPLC-MS/MS, which\nwere then compared between the two groups. An appropriate high sensitivity and selectivity ultraperformance liquid chromatography-\ntandem mass spectrometry (UPLC-MS/MS) method was applied to the comparison of plasma pharmacokinetics in\nMG and MG-Na using carbamazepine as internal standard (IS).............................
In this study, a sensitive and reliable HPLC-MS/MS method was established to quantify tamarixetin in rat plasma. This method\nwas then applied to research on the pharmacokinetic and bioavailability of tamarixetin after intravenous and oral administration\nin vivo....................................
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